Markterkundung - Automatisiertes Mikroskop High-Content-Screening (HCS)

Das Max-Planck-Institut für molekulare Zellbiiologie und Genetik beabsichtigt einen Kaufvertrag für ein Automatisiertes Mikroskop für High-Content-Screening (HCS) ggfs. inklusive Servicevertrag bis zu 4 Jahren abzuschliessen. Mandatory Requirements: - Light Source: At least 4 laser excitations ranging from 405nm to 640nm covering the spectal range for 4 color imaging, LED light source for transmitted light/bright field imaging/phase contrast; -Detection system: 3-4 digital sCMOS cameras with high quantum efficiency (max at least 80%) in a geometry that allows simultanous image acquisition of 3 channels. Background noise should be below or around 100. The camera set up should have ate least 2000 x 2000 pixels and allow for different modes for binning (1x1, 2x2, 4x4); - Timelapse modality: should allow high speed timelapse modality: ideally >=40 fps (bin 1); - Spectral discrimination: Dichroic mirrors and filter that allow multi-color, fluorescent imaging of the typical dye spectrum, e.g. 405, 488, 568 and 647nm; - Objectives: High quality, achromatic objectives ranging from ~4x to at least ~60x, water immersion objectives for 20x to at least 60x, e.g. 4x (NA at least 0.1, working distance at least 5 mm), 10x (NA at least 0.35, working distance at least 2.5 mm), 20x (NA at least 0.8, working distance at least 0.5 mm), 40x (NA at least 0.9, working distance at least 0.2 mm), 60x (NA at least 1.1, working distance at least 0.25 mm). In addition, high quality, high working distance (5 mm), achromatic objective phase contrast 20x (NA at least 0.45); - Autofocus: Laser-based automated autofocus; - Confocality: Dual-disk spinning disk system with ~50 µm pinhole size (or equivalent) for confocal microscopy: Z axial resolution should be at least 15 µm for a 10x objective, 3 µm for the 20x, 1.5 µm for the 40x, and 1.0 µm for the 60x; - Environmental control: stage incubator for temperature control (~37°C), CO2 control (~5%), O2 Control (~1%) and humidified atmosphere ; - Imaging modalities: Control software of HCS microscope to set imaging modalities such as multi channel (parallel), z-stacks, time courses, fast live cell imaging (at least 10fps, ideally 40fps binning 1), specific well and sub-well layouts. The software should allow "smart microscopy" features using a first a low magnification imaging, then an image analysis and second a higher magnification "guided" imaging. This is particularly important for sparse objects. Usage of third party image analysis software should be possible; - Vessel support: Support of various micro titer plated like, 6, 12, 24, 48, 96, 384, and 1536 well plates, plastic as well as glass bottom, slides in slide holder; - Barcoder reader for tracking plates; - Automation: Automated robotic plate handler with plate hotel (RT) and incubator (temperature 10°C to 40°C, CO2) integrated with scheduling software to control HCS reader allowing 24h/7d imaging; - Computer workstation with addional data storage discs (SSD) and keyboard, mouse, and 2 large monitors; - Dimensions: The entire system including the automation should not exceed the following dimensions: 300 cm width, 130 cm depth, 200 cm height;